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This start arm insert covered approximately 60% of the start arm and resulted in a 12 cm discontiguity between the start arm and goal arm floor coverings (Fig. 4A).
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The prior Bernoulli probability of event S, whereby an insert covers a possible site of SV, is conditioned upon its length.
It must be noted that the package insert covers adverse events observed with bortezomib monotherapy only, which does not require pre- and/or post-hydration and represents a minimal fluid load.
In invasion assay, the similar inserts covered with Matrigel (2 mg/ml; Becton Dickinson) were used to measure the cell invasion ability.
Ishiwaka and Hec-1A cells (6×10) were seeded 24 h before serum deprivation for 24 or 48 h, respectively, and 2×10 cells were seeded over 8 μm-porous inserts covered with matrigel (Matrigel Invasion Chamber 8.0 μm, BD Biosciences, Bedford, USA).
Pendo recently raised a $25 million Series C, partly to fund acquisitions and international expansion — it looks like the Insert deal covers both.
mv microvilli Fig. 8 Transmission electron micrographic images of the alveolar-like structures observed in cultures of AECs in direct contact with BMSCs on day 14. a Alveolar-like structures consist of mesenchymal cells and matrix on the culture insert (asterisk) covered with an epithelial cell monolayer.
Ideally, the length of these amplicons is not much larger than the average read length, to ensure that most bases in the amplicon insert are covered by reads in either direction.
Briefly, the membrane of each insert was covered with 100 µl of ECM gel (Sigma Aldrich, St . Louis MO) and the cells were kept in serum free medium (DMEM F12+1%1% penicillin/streptomycin + 1% Amphotericin B) for 24 h. 2 × 10 hESC from women without endometriosis and with endometriosis with WT or variant KRAS alleles were seeded into the inserts and incubated for 48 h.
The sites where the tantalum beads had been inserted were covered by fibrin-glue.
The finished full-length sequenced inserts were covered by an average of 4.1 reads (total number of bases sequenced/total number of bases in all FLIcs).
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