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In comparison, the cold insert counts normalized to warm background in the 18F phantom image was 26%.
At the highest 90Y activity scan, cold insert counts was approximately 33% of warm background, much closer to that of 18F.
The median cold insert counts percentage across 40 PET imaging volumes was 45.3% of the true warm background activity concentration (mean 48.8%, range 23.9 104%).
The median cold insert counts (C i ) increased from 33.3 to 75.6% in the lung insert from 0.29 MBq/ml to the lower true activity concentrations of 0.02 MBq/ml.
Cells from a minimum of three high power fields per insert were counted under light microscopy (Olympus, Solna, Sweden).
After incubation for 18 h at 37°C, the filters were stained with Diff-Quick stain set (Dade Berhring Inc., Newark, DE), and the number of cells that penetrated the Matrigel and the control insert were counted with a microscope using a 20× objective (5 randomly selected fields).
Four fields of view from each insert were counted.
Cells on the bottom of the insert were counted according to the manufacturer's instructions.
The number of invaded cells attached to the other side of the insert was counted from 9 random fields.
Membranes were dried, and numbers of invasive cells on each insert were counted by viewing under an Olympus AX70 microscope.
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CEO of Professional Science Editing for Scientists @ prosciediting.com