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Design of final synthetic insert containing restriction sites in both ends was done and submitted to GenBank.
Although prescriptions come with a lengthy package insert containing detailed information about the drug, the information is often written in technical terms and printed in microscopic type.
Instead, and quite beautifully, you get a sketchbook insert containing Julie Taymor's costume designs for "The Lion King," reproduction pages from the "Tarzan" prompt script and many other pull-out, pop-up or accordion-fold extras.
An exact match was found with an insert containing an open coding region for the full-length candidate 1,25D3-MARRS 1,25D3-MARRS
Results: A bacterial artificial chromosome clone with an approximately 100-kilobase insert containing nearly the entire human NF2 gene has been isolated.
The insert containing the two AcrA KO fragments flanking the floxed neomycin resistance cassette (Supplementary figure 4A) was introduced into P. pallidum cells, and gene knock-outs were identified by PCR and Southern blot (Supplementary figure 4B,C).
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Insert-containing constructs were then transformed to Origami B expressing bacteria.
Insert-containing clones were identified by agarose gel electrophoresis after plasmid EcoRI digestion.
A total of 106 insert-containing clones randomly selected from the library yielded 227 unique sequences, which indicated that each concatamer contained ∼2 small RNA sequences.
Colonies were picked and analyzed for the insert-containing plasmid by direct PCR with the vector primers M13F and M13R followed by gel electrophoreses of the amplified products.
To this end, we constructed a library of size-fractionated RNAs from S. japonicum adult worms and analyzed 227 RNA sequences from a total of 106 insert-containing clones randomly selected from the library.
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