Sentence examples for insert constructed from inspiring English sources

Back online, I next considered the Rev-a-Shelf Wooden Spice Drawer Insert, constructed of birch and maple, which slides into a drawer to hold three rows of spices.

None of these sequences tested yielded Photinus luciferase activities (encoded in the second cistron) higher than the negative control (RP; no insert) construct.

For preparation of the sense insert, construct pGEXKG/PKV [ 33] was doubly digested with BamHI and SstI, and the PKV sense fragments were recovered.

In the present design, the recombinant SpRNAi-RGFP gene expresses an intronic insert construct reminiscent of a hairpin-like pre-mRNA structure.

Every RIG-I, MDA5 and MeV V insert construct after subcloning in the expression vector was entirely verified by sequencing (Eurofins, Ebersberg, Germany).

To prepare the antisense insert, construct pET28a/PKV [ 33] was first digested with EcoRI to release the PKV insert, which was then ligated back into the pET28a vector at the EcoRI site, generating clones with opposite orientations.

To ensure that the inserted construct is transcribed as expected, the gus linker expression was examined using RT-PCR.

For the MON 810 amplicon transgenic leaf material (100%) was used, with a 2 1 ratio between the invertase and the inserted construct.

To discard the presence of a wild-type copy of Ppα-DOX adjacent to the inserted construct in Ppα-DOX-GUS-2, PCR amplification was performed using primers DOX3F+3′DOXr.

The same principle applies to computational subtraction using genomic DNA, but here the size of the inserted construct relative to the target genome will be the most important factor [ 11].

All our modules were integrated at a transcriptionally "neutral" site in the yeast genome (Flagfeldt et al. 2009), where the gene expression of the inserted construct was the highest compared to other genomic sites investigated.