pNCBgl-NN contains a 3.2 kb insert comprising the promoter, coding region, and terminator of the N. crassa pyr4 gene isolated from plasmid pFB6 [ 32] and a polylinker with unique NheI and NotI sites located at the 3'end of the pyr4 insert.
The library inserts comprised homologous regions to the BamHI/NotI-digested pYD1 backbone on both sides in order to facilitate gap repair driven homologous recombination in S. cerevisiae.
The twenty large genomic tracks 30-522 kb) captured as fosmid inserts comprise gene regions with roles in flowering, disease resistance, and metabolism.
showed higher water uptake ability (P < 0.05) as compared to inserts comprising low concentrations of CH (F1, F2; CH : CS 30 : 70, 40 : 60, resp .. Swelling is a function of the presence of ionized functional groups.
Eleven tandem copies of a 672 nucleotide insert comprise a region of the chromosome that challenged the correct assembly of the genome, and finishing this region was the final step for the sequencing phase of this project (see Methods).
The βB-sheet insert is comprised of three antiparallel β-strands: the β-1-strand (residues 48 52) at the N-terminal end; the novel βB-strand (residues 104 109); and the β9-strand of the activation segment (residues 215 217).
Insert 7416_13 comprised 67 bp unique sequence from chromosome 10 120,819,239-120,819,305, plus polyA and duplication of AAGAAATATTTCCC.
In this study, we produced a large plasmid that contained a 5, 10, or 20 kb insert, which comprised 10 segments in defined orientations and a defined order.
Clones were determined to lack inserts if ≥ 200 bases from the 5' end of the EST were identified as vector or if the insert was comprised of fewer than 100 bases of non-vector masked sequence.
Soluble ocular drug insert matrices comprised of raw gelatin [65 68] or chemically modified [69,70] and composite gelatin [71,72] gels also indicate the feasibility of these biomaterials for LEC culture.
