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In contrast to palatal implants, mini-screws are often preferred because they are less expensive, are easier to insert by the orthodontist without the need for the oral surgeon and can be loaded immediately.
The rest of the tungsten carbide surrounded the sub-critical mass target cylinder (called the "insert" by the designers) with air space between it and the insert.
In this case, the two clones could not be used to extend the insert by the double crossing-over recombination.
The p.R435C mutation was introduced in the pLEICS-23 plasmid, which was carrying a mCherry-tagged LMNA insert, by the PROTEX cloning service of the Department of Biochemistry, University of Leicester.
The results were similar to those seen with the empty vector, but in this case, we also assayed Cu2+-inducible expression of mCherry in clones that contained only a correct insert by the attL/attR/attB test.
For other EST sequences, such as in condition B, the entire vector fragment, extending from the base numbered 1 in its conventional expression to the ligation site, was mistakenly recognized as part of the cDNA insert by the SeqClean program and remained untrimmed by SeqClean processing.
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Upon verification of the DNA insert by sequencing, the construct was used to transform Col-0.
An LMA is conventionally inserted by the index finger insertion technique as described by Dr. Archie Brain.
Sequences were compared to the T-DNA insert by using the tool 'bl2seq' [ 34].
Inserted by the Story Dept.
All nails were inserted by the retrograde technique.
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