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Transformation of Arabidopsis with 35S::CEL9B3SP: ScAGU115 resulted in 15 independent homozygotic lines that contained a single insert as indicated by the segregation analysis in T2, and 14 of those lines expressed the transgene, as found with RT-PCR (Additional file 2: Figure S2A).
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The extra atomic planes are inserted as indicated by arrows.
Subdermal needle electrodes were inserted as indicated above.
Statistical significance was assessed as indicated.
Test, publish, review and change as indicated.
The vector for expression of GST-elafin (a.a. 61 to 117) was obtained by PCR amplification of the DNA coding sequence of trappin-2 (pre-elafin) with specific primers and using the pGE-SKAP plasmid (gift of Thierry Moreau, Tours, France) as a template, and the PCR product was inserted into pGEX4T-1 as indicated above.
The X indicates the position of the inserted stop codon, as indicated on the sequence alignments in Fig. 2 (A).
Some micro-structures on epidermis cells arise from sub-cuticular inserts of mineral crystals, as indicated in Fig. 17b.
It inserts by homologous recombination, as indicated by the absence of target site duplication, and causes rearrangements.
The 5c-g insertion appeared to be specific to Saccharomyces and indicated that at some point during evolution this piece of the RNA was inserted near the 5' end as indicated in Fig. 4. The evolution of Saccharomyces also involved the enlargement of helices 5h and 5i.
x -axis indicates the insert as well as what refractory period group each male was placed in.
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