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The DNA sequence of the insert as well as the flanking regions in each cDNA clone was verified by sequencing.
The DNA sequence of the insert as well as its flanking regions in the plasmid construct was verified by sequencing.
The respective prey and bait constructs with a different insert as well as the empty vectors were co-transformed into the yeast strain, PJ69-4A, to detect the interaction.
x -axis indicates the insert as well as what refractory period group each male was placed in.
On the humeral side, the depth of the polyethylene insert as well as the neck shaft angle could be varied.
The pETM41 vector encodes a maltose-binding protein (MBP) N-terminally to the insert, as well as a C-terminal His-tag.
In addition, this vector family permits easy HA-tagging, sequencing and in vitro expression of the insert, as well as the production of retroviral particles if needed.
The plasmid and insert as well as some of the PCR-amplified probes were labeled according to the Invitrogen BioNick labeling system.
Human Cot-1 DNA and plasmid DNA without insert as well as a hybridization mix without DNA served as negative controls for hybridization.
However, HDAC inhibitors typically function through chelation of the active site zinc ion, and the DAC3 insert, as well as the other differences, may have an impact on access to the active site pocket for both substrates and inhibitors.
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