We also used E. coli assays to show that group II introns could be used for genetic repair, either by inserting a functional copy of the gene into a defective gene, or by inserting functional exons preceded by a splice acceptor site to circumvent defective downstream exons [43].
This involves inserting a functional copy of the gene associated with the disease into an inactivated virus, which is then injected into the eye.
For that purpose, we identified the site at which a loop containing the recognition sequence for cre-lox recombinase could be inserted yielding a functional enzyme.
Again, this suggests that the entire cluster can be excised or inserted as a functional unit.
The achievement of new biospecific organised ultrathin films, directly interfaced with the transducer and inserting biomolecules in a functional and orientated position, may open an original way in the development of new biomimetic optoelectronic devices.
After normalization to E1a-neo RNA, E1b-Luc RNA expression in the cell lines containing an inactivated termination sequence (DPM) was increased over the levels obtained from inserted sequences with a functional terminator (GGT) (Table 3).
The interfacial structures, playing a critical role on the transport properties and the perpendicular magnetic anisotropy in thin films and multilayers, can be modified by inserting an ultrathin functional layer at the various interfaces.
However, although only 10% of LAR mRNAs contains the B insert, knockdown also revealed a functional role of LAR as a SALM3 interactor, perhaps through high protein level, surface expression, or affinity, as suggested by the cell-based SALM3-Fc binding assay using equal amounts of LAR-RPTP expression vectors.
To this alignment, we added the GH11 sequences obtained by the random sequencing of plasmid inserts, the sequences producing a functional enzyme in yeast and the sequences representative of the most abundant Illumina sequence clusters before (H0) or after (H1 and H2) SHS capture.
