Sentence examples for insert a fragment from inspiring English sources

An AflII site approximately 60 bp from the 3′ end of the open-reading frame was used to insert a fragment encoding 3 HA tags in frame, created using oligonucleotides GT179 and GT180.

The system described here uses the ability of Flp to catalyze two intermolecular reactions (or perhaps one intermolecular reaction followed by an intramolecular reaction) and thereby to insert a fragment into the receptor plasmid.

pAAV2neo was constructed by inserting a fragment containing two inverted terminal repeats (ITRs) from AAV2, the CMV promoter, multi-cloning sites, and BGH polyA into pSV2neo [18].

The RNAi vector of OsDR10 was constructed by inserting a fragment of cDNA digested from cDNA clone BI71N2 using restriction enzymes KpnI and BamHI into the pDS1301 vector (Figure S2B) [37].

A luciferase reporter construct, psi-FGFR1, was constructed by inserting a fragment of FGFR1 3′-UTR that included miR-133a/b and miR-214 binding sites into the luciferase reporter vector psi-CHECK-2.

The strain MK-ER-Ndt80 (inducible Ndt80 by estradiol, see Carlile et al [ 29]), was made by inserting a fragment of the plasmid PKB80 into the ura3 locus (selecting for Ura+ transformants).

The h TERC expression vector pSSP-hTERC was constructed by inserting a fragment containing h TERC in the pSSP plasmid (Salzano et al. 2009), carrying the puromycin resistance gene, under the control of the U1 promoter.

To test in vitro whether the methylation of the P2 promoter affects IGF1 gene expression, we used a reporter gene construct made by inserting a fragment of IGF1 promotertextendinging from −1,014 to +64 bp of the TSS - thus containing 7 of the studied CGs - into a promoter-less CpG-free luciferase expression plasmid.

To investigate whether the contribution of miR-204 to the enhanced killing of GTL-16 upon treatment with 5-fluorouracil or oxaliplatin occurs, at least in part, through the targeting of Bcl-2 expression, we inserted a fragment of 541 base pair of the Bcl-2 3′-UTR conthreeng three miR-204 binding sequences downstream of the luciferase coding regions (Supplementary Figure 3e f).

The full-length human RIP3 cDNA was cloned into pFastBac HT B plasmids (Invitrogen, Grand Island, NY, USA), which was modified by inserting a fragment of DNA sequence encoding GST into the upstream of the multiple cloning sites of the primary plasmid.

Although whole clones are employed in some hybridisation studies [ 3], usually only the insert or a fragment of it is used as a probe.