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In the lower part of the box, next to "When you insert a CD," select "Import CD and Eject".
Rather than requiring users to load their own music into an online locker, which can be a time-consuming process, My.MP3.com allowed them to simply insert a CD into the CD-ROM drive of a computer.
If you have a broadband connection like D.S.L. or cable, it might be faster to use a Web version of an encyclopedia than to find and insert a CD or DVD.
Rather, a user had merely to insert a CD into the CD-ROM drive of a computer and log into MP3.com's Web site, at which point MP3.com would automatically put a copy of the music into the person's virtual locker.
And if you think that the Beatles have an airtight operation, think again: the sampler's entry at Gracenote (that magical entity, formerly known as the CDDB, that calls up track names when you insert a CD into your computer) is riddled with errors: "I Am the Warlus," "Moter Nature's Son," "Grass Onion".
It plays songs downloaded only from Apple's iTunes site, unless you insert a CD; no other downloads welcome.
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A similar experiment with two primer pairs VRARDHIS and VRARIHIS was set up to remove the N-terminal TEV protease cleavable His-tag and insert a C-terminal His-tag in a single reaction.
Since fluorescently tagged γ-tubulin fusions were not fully functional (not shown), we used a CRISPR/Cas9-mediated strategy (Dickinson et al., 2013) to insert a C-terminal GFP tag in the endogenous locus of gip-2, which encodes an essential component of the C. elegans γ-tubulin complex (Hannak et al., 2002).
This receptor was engineered by inserting a C-terminal rho1D4 epitope tag and an N-terminal FLAG epitope tag to allow its purification and detection.
To create a control strain, we inserted a C-terminal 3ha tag and a TRP1 selection marker at the RPN4 locus in the parental YFP17 strain.
However, whereas yeast and human Polη replicate through 8-oxoG by predominantly inserting a C [25], human Polκ is more efficient at inserting an A opposite the lesion than a C [26].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com