Whether by taking business calls with a bright yellow phone headset, drinking what Honey Boo-Boo calls 'Go Juice', or inserting a wacky cat photo into a company presentation, O'Leary definitely doesn't fit the humorless mold of who you might expect to be heading up customer relations at an enterprise software firm.
And it is probably safe to say that until this moment it had not seen the likes of a rainbow-striped sweater with lace inserts, embroidered with a cat sporting a spiked halo of the type seen in early Christian art, teamed with a kilt in the mustard yellow check made famous by Alicia Silverstone in the film Clueless, finished off with lace tights and spike-heel boots with seven silver buckles each.
To set up a culture system with two compartments, the inner wall of a culture insert (Ibidi, cat. no. 80209) was removed and the modified insert was placed in the middle of a 60 mm cell culture dish.
The team, headed by Mark Westhusin, removed the nuclei of cells from the lining of a cat's cheek and inserted them into enucleated cat eggs.
"That's not sexy to me," Robinson Pete inserted before asking, "have you ever met a cat who doesn't know sports, like you know sports more than him?
If you know your cat likes catnip, insert a small amount into the stuffed animal.
For immunofluorescent analysis of SVEC4-10 cells, the cells were grown on 12-mm Transwells with 0.4 μm Pore Polyester Membrane Insert (Corning, Cat. no. 3460), as described in Section 2.3, until they reached confluence, and then were washed with PBS and fixed in 3.7% paraformaldehyde for 10 minutes at room temperature.
However, failing this you may need to insert the cat into the box, which can be tricky.
For donor strain we used S. Typhimurium FSL R8-3980, whash has a chromosomally inserted cat gene (chloramphenicol resistance); as recipient we used the wild type strain FSL R8-3981.
