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In these cases, the best match is often an insect sequence suggesting small amounts of contaminants in the cDNA libraries.
If there were more than two species in either of the 5 species sets (except for insect set) showing higher score and identity value than the corresponding top bacteria hit in blast-I, the insect sequence was deleted from candidate gene set because of its possible close relationship to gene in other eukaryotic species rather than bacteria.
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The honey bee is the fifth insect sequenced, and 170 researchers worldwide have had a hand in interpreting its 10,157 genes.
Indeed, N. vectensis sequences appear, along with B. floridae and C. teleta sequences, as a sister group of the vertebrate and insect sequences, in contrast to T. adhaerens, S. purpuratus, and C. intestinalis sequences that appear much more divergent.
Nearly 85.9% of the C. lectularius sequences showed similarity to insect sequences, but 44.8% of the deduced proteins of C. lectularius did not show similarity with sequences in the GenBank non-redundant database.
Several insect sequences were downloaded to represent the diversity of insect lysozyme sequences.
Insect sequences with no similar gene in other insects were recovered as candidate sequences.
In the second filter, the insect sequences in these orthologs were removed except B. mori.
The branch lengths separating the annotated insect Dscam sequences (clade I) from clades II (vertebrate sequences) and clade III (unannotated insect sequences) are roughly comparable.
However, both crustacean and insect sequences clustered into the third group containing the insect ferritin 3 genes.
Most of the M. tardigradum AQPs formed a clade outside of the insect sequences together with nematode sequences.
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CEO of Professional Science Editing for Scientists @ prosciediting.com