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Participants were asked about their lifestyle and insect exposure.
The extent of insect exposure on the Bellarine Peninsula is shown in Table 3.
Those from Sogan-Godud were more likely to have used mosquito nets (odds ratio [OR] 5.2, p = 0.0001) and mosquito coils (OR 8.2, p = 0.0001) to reduce insect exposure.
Case-patients and control participants were mailed a written questionnaire with questions about their medical history; outdoor lifestyle and behavior; and soil, animal, and insect exposure on the Bellarine Peninsula.
Both represent challenges in rural Africa where resources for construction are limited and closing windows and doors at night for safety and/or to reduce insect exposure is common.
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"Contamination" encompasses contaminated food or water, contamination from insects, exposure to a "dirty" environment, or poor personal hygiene (of caretaker or child).
Although the responses to our insect-exposure questions suggest an association between mosquito bites and M. ulcerans, this association may be just a nonspecific marker of environmental exposure.
Most conventional insecticides act very rapidly and it is unlikely that the induction of detoxification enzymes will play an important part in determining the fate of the insect upon exposure to a pyrethroid-treated net.
We also found overlap among genes that were constitutively over-expressed in resistant insects with genes that were up-regulated in susceptible insects after exposure to Xentari™, suggesting a permanent activation of the response to Xentari™ in resistant insects.
Results show strong over-expression of many genes that were also found up regulated in susceptible insects after exposure to sublethal concentrations of Xentari™.
After various time intervals of exposure, insects were removed and the insect tissues were dissected as described above for enzyme assay.
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