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They were obtained from insect cultures kept in the insectary of Faculty of Agriculture and Veterinary Medicine, An-Najah Nablusal University, Nablus, Palestinian Territories.
Thanks to the Singer lab group for helping maintain the insect cultures used in experiments.
Insect cultures (culture boxes) were kept under constant laboratory conditions (24±1°C, 70±10% Hr, DL 12∶12).
Thanks to A. Gassmann, T. Dennehy, Y. Carrière and C. Ellers-Kirk (U. Arizona) for helping obtain source insects for our insect cultures.
WJM performed insect selection, carried out the insect cultures and participated in the manuscript preparation.
We thank Rajanna TS and Nagarathna M for maintaining insect cultures.
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Influenza vaccine cultured on human cells or insect culture are not approved for pediatric use.
Briefly, promastigote forms were cultured in 24-well plates in Grace's insect culture medium (Sigma) supplemented with 20% heat-inactivated FCS (Cripion, Andradina, Brazil), 2 mM L-glutamine, 100 U/ml penicillin, and 100 μg/ml streptomycin, at 26°C (6 days).
The present study focuses the impact of various stirrer configurations, sparging rates, as well as 2 different bioreactor scales on micro-bubbles in SF-900 II insect culture medium, which represents a suitable example for Pluronic F68 supplemented culture media commonly applied in cell culture processes.
To obtain a mass production of S. cerealella eggs, the insect culture was maintained according to the method described by Hassan (1995) with some modifications where a bulk of soft wheat grains were brought to the laboratory and were sterilized at 120 °C for 2 h.
The insect culture media was filtered before purification.
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