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For this purpose, the raster layers of the groundwater quality factors or the neuro-fuzzy network inputs were prepared in GIS with the similar pixel sizes (1 × 1 m) and then were combined with each other.
Six sets of 100 inputs were prepared.
Similar(58)
Inputs are prepared by fixing the initial states of a quantum dot molecule, and outputs determined by reading its value at a given time T later.
Five 3-fold serial dilutions of the input were prepared and quantified by qPCR along with the immunoprecipitated DNA.
Five to fifteen nanograms of ChIP DNA or un-enriched whole cell extract (Input) were prepared for sequencing on an Illumina HiSeq2000.
Two sets of libraries (ChIP and input) were prepared for each of the cell lines from samples immunoprecipitated on separate occasions.
Briefly, two-color labeling reactions (300 ng-3 μg total RNA as input) were prepared by using Agilent Two-Color Quick Amp Labeling kit (version 6.0, Agilent Technologies) following manufacturer's protocol.
Samples that met the Illumina sample input guidelines were prepared according the TruSeq® ChIP Sample Preparation kit (Illumina Inc., San Diego, California, USA) with minor modifications.
Input files were prepared using RspMatchEDT a product of GeoMotions http://www.geomotions.com.
Therefore, raster layers of the three input parameters were prepared and those were combined using overlay analysis with a pixel size 1 × 1 km (similar pixel size).
Input structures were prepared by using two different methods: (i), using Gasteiger charges for both the ligands and the proteins [17]; (ii), using PM6 charges calculated by MOPAC2009 [70] for both the ligands and the proteins.
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