Sentence examples for inputs were detected from inspiring English sources
Immunoprecipitated proteins and inputs were detected by western blot analysis using: rabbit anti-flag (Sigma), mouse anti-V5 (Abcam) and mouse anti-GAPDH (Abcam) primary antibodies and goat anti-rabbit (Abcam) or goat anti-mouse (Sigma) secondary antibodies.
Due to this channel dependency the algorithm will update itself every time a new input is detected in a new channel, or if the spectral content of an input channel suffers from a drastic change over time.
To modify this mechanism to fit our hypothesis would require the K cells to positively gate all the inputs to the stellate cell when the thalamic input was detected but a stellate cell output was not detected.
The input proteins were detected by Western blot.
At this threshold, 51 of the 65 previously known Drosophila miRNAs covered by the multiz9 and 51 of the 76 covered by the multiz15 input alignments were recovered (37/51 miRNAs were detected in both inputs).
If no such candidates were detected, user input was requested in identifying the ROI in the current frame.
However, much lesser amount of HIF-2α was precipited in IMR-32, where only faint bands were detected for input of both HIF-αs.
The data were examined to determine if any probe consistently over or under-measured its corresponding input percentage and no biases were detected (data not shown).
To prepare cell lysates, cells were suspended in a PCR lysis buffer containing 50 μg/mL proteinase K and, following the inactivation of the proteinase K, the input and replicated viral DNAs were detected by real-time PCR as described previously.
At least two independent ChIP experiments were performed to confirm the binding of AP-2γ to the four isolated ChIP fragments near to genes ErbB2, CDH2, HPSE and IGSF11, the related PCR products were detected from input samples and AP-2γ-ChIP-derived DNA samples, but not from IgG-ChIP-derived DNA samples.
Similar amounts of GFP and GAPDH proteins were detected in the input extracts (Fig. 3B).
