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The fitness equation r = kC(b e−Lr −1) was taken from Bull (2006) and required inputs of cell density (C = 1 × 10/mL), adsorption rate (k = 5 × 10−9 mL/min), lysis time (L = 19 min), and burst size (b = 300) to calculate the rate of growth, r, which when multiplied by 60/ln(2) gives doublings/hr, the measure of fitness used here.
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This formalism can be used to describe the permutations of inputs of cells as in [135, 136].
Lane 3 corresponds to the input of cells expressing both proteins (20% of the total lysate) before the ip.
Proof-of-principle encapsulation devices often assume an unlimited input of cells, which is not realistic except in the cases of abundant primary cells or cultured cells.
Thus, each villus tree is likely derived from the cells present in the initial villus bud without further input of cells.
The model also includes thymic resting/naïve T cells (Rr and Er, Figure 4A) arising from a discrete stochastic input of cells in the immune system, mimicking the apparently random nature of the pulse trains of resting cells (which can become activated by antigen presentation).
Input 10% of cell lysate for IP and 50 g proteins were analyzed by Western blotting.
We observed, with high-precision, approximately three orders of magnitude of linear dynamic range for a cell input of 0.24 – 250 cells per μl (intra-assay %CV < 1.1 %) (Additional file 1: Figure S1A).
The inverse of this quantity provides an alternative estimate for the number of inputs to cells in a given age group.
The objective is to minimize the tumor cells for a given input of dendritic cells.
These synaptic potentials arise from the activity of thousands of inputs that each cell receives on its cell body and dendrites.
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