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Although the presented method aims to provide an assessable platform for researchers, MOCCA can be integrated into microfluidic chambers with inputs for cell medium and oil to facilitate automated, high-throughput analyses.
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To overcome this limitation, we sought to develop automated cell type recognition that uses the descriptors as an input for cell type classification.
The utilization of input energy for cell rupture reaches its maximum if the disintegration rate correlates with specific energy.
Therefore, the application of protease prior anaerobic digestion of C. vulgaris could be a promising approach to decrease the energetic input required for cell wall disruption.
Cell extracts were resolved by SDS-PAGE and immunoblotted, with the respective antibodies, as previously described [ 23]. β-ACTIN was used as input control for cell lysate.
Glucose and glutamine are the two major nutrient inputs for cancer cells as they provide bioenergetics and intermediates for macromolecular synthesis in proliferating cancer cells.
The characterization of input material for cell-based therapy bioprocesses from multiple donors is necessary to identify and understand the potential implications of input variation on process development.
(D ) Average levels of input HOTTIP RNA for cell-based native RNA immunoprecipitation assays of WDR5 mutants by qRT-PCR, relative to GAPDH.
It could be shown tha the disintegration of the investigated organism is a function of specific energy input for high cell concentrations in the suspension together with bead sizes between 0.2 and 0.8 mm.
Given that chemoattractants activate PI3K, this provides a strong argument that endogenously present PI3K enzymes are sufficient as a physiological input for triggering cell polarization and migration.
The numbers of Lin−Sca1+cKithiGFP+ cells were adjusted after 48 h infection to obtain equivalent numbers of wild-type (WT) and Jnk-deficient, GFP− and GFP+ LSKs in the inoculum (25% input for each cell type).
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