ADT tool was used to prepare parameter and input files as previously reported (Huey et al, 2007; Li and Li, 2010).
The relative intensities of the amplified products were normalized to the input DNAs as previously described (Lee et al. 2003; Sullivan et al. 2007).
The issue of potential differences in quantity of sequence reads between indexed samples due to the efficiency of indexed adapter ligation to input DNA, as previously reported by Craig et al., was addressed in this study by multiplexing the indexed in equal quantities pre-enrichment.
Results were quantified by qRT PCR using the primers listed in Supplementary Material, Table S3, and the enrichment of DNA was calculated in terms of percent of input, as described previously (46).
While PLSOM exhibits a lower mean (E{^{prime }}(t)) on all data streams, except Gauss, it does so at the expense of not mapping the input space density, as previously demonstrated.
The PCR primer sequences used for analysis of Xnot, Xpapc and ODC, an internal input control, were as previously described [35], [36] [http://www.hhmi.ucla.edu/derobertis/].
The PCR primer sequences used for the analysis of Xnot, Xpapc, and ODC, an internal input control, were as previously described [30], [31] [http://www.hhmi.ucla.edu/derobertis/].
20 ng of each sample was used to confirm enrichment of methylated locus (OXT) and a concomitant depletion of un-methylated locus (UBE2B) versus the input by qPCR, as previously described [ 21].
Acute arthritis and associated nociceptive stimulation might lead to increased proprioceptor thresholds thus gating the proprioceptive inputs, as it was previously reported by Rossi et al. [ 13] during locomotion in the case of foot pain.
These glycinergic neurons, like all other CN neurons, receive functional inputs from PN axons, as previously suggested by immunohistochemical and optogenetic studies (De Zeeuw and Berrebi, 1995; Teune et al., 1998).
