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Immunoprecipitated products and corresponding input were assembled into a library using Illumina protocols.
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Then the final tables of inputs, costs, yields, and investment returns were assembled and analysed to examine trends for each year that this benchmarking exercise was performed--2005 performed--2005 performed--2005
Using an in-house Perl script, make_gen, the genotype data and the locus names were assembled into CRI-MAP input format.
The sequences retrieved were assembled with the sequence assembler software Newbler v2.3 (Roche) http://my454.com/ with default settings for cDNA assembly with the sff-files as input file.
The reads were assembled into consensus sequences using Rnnotator 2.4.14 [ 147], which takes short read sequences as input, and outputs assembled transcript contigs.
Images were assembled using Photoshop.
Chromatograms were assembled in DNASTAR Lasergene SeqMan Pro v.7.1.0.
Four transcriptomes were assembled.
92,511 scaffolds were assembled.
To develop and verify the models a large database that covers the complete range of potential input conditions was assembled.
To construct the MspI and ApeKI digested RRBS library, 100 ng of input genomic DNA was assembled into 100 μl of reactions with 300 units of MspI (NEB) in 1× NEBuffer 2, incubated at 37°C for 7 h, and then 80°C for 20 min. Twenty units of ApeKI (NEB) were then added and incubated at 75°C for 16 20 h.
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