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Ions could diffuse laterally at the interface when strong input was loaded.
Typically, about 10 to 20 times more precipitate than input was loaded into the gel.
For detection of proteins by Western blot, 5 µl of each input was loaded for each blot (approximately 0.5% of material used in IP).
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Protein extracts (10% of input) were loaded next to samples subjected to the entrapment assay.
These inputs are loaded and stored in PREESM by the SDF4J library.
The immunoprecipitate together with input lysate was loaded to Mini-Protean TGX 4-20% SDS-PAGE gel (Bio-Rad).
1% to 2% of the initial homogenate was loaded as input.
10% of each total lysate was loaded as input and 25% of each bound sample was loaded for each Western blot analysis.
Ten percent of the total protein subjected to immunoprecipitation was loaded as input and 30 μl of the remaining supernatant after immunoprecipitation was also loaded to confirm immunodepletion.
The required input of 500 000 enriched beads was loaded onto the Pico Titer Plate (PTP) and sequencing was carried out for 200 cycles using full processing mode for amplicons (GS Junior Sequencer, 454/Roche).
For input 10% of final IP sample was loaded.
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