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For example: "there is still an undercurrent that questions the issue of the added value of patient input" was labelled as "(low) expectations of patients' contribution".
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Input was labeled with Cy3 dye, while immunoprecipitated samples were labeled with Cy5 dye.
Two micrograms of each amplified MeDIP and input were labeled for each slide of the 2-slide set.
Two fractions from each sample (MeDIP enriched and input) were labeled with Cy5 and Cy3, respectively, and cohybridized to the NimbleGen Human DNA Methylation 2.1M Deluxe Promoter Array (Roche/NimbleGen, Madison, WI, USA).
Under the traditional training scheme, these inputs are labeled with hard targets and the network must learn a function that is highly nonlinear in this region.
Granule cells with more than two ECN inputs were labeled with blue crosses (E + granule cells).
Granule cells with more than two BPN inputs were labeled with blue crosses (B + granule cells).
Input DNA was labeled with Alexa Fluor 647 and the immunoprecipitated DNA with Cy3.
A second round amplification of 15 cycles was added to increase the yield of DNA. 3 µg of amplified ChIP DNA and Input DNA was labeled with Cy5– dUTP and Cy3-dUTP, respectively, with a BioPrime DNA Labeling System (Invitrogen).
Fragmented (input) DNA was labeled with Cy3, and enriched (methylated) DNA was labeled with Cy5, using the NimbleGen Dual-Color DNA Labeling kit (Roche) according to the manufacturers' instructions.
Briefly, 1 μg IP or input DNA was labeled with Cy-dye random nonamers (Trilink Biotechnologies, CA, USA) using Klenow fragment (New England Biolabs, MA, USA), precipitated, and resuspended in hybridization buffer.
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CEO of Professional Science Editing for Scientists @ prosciediting.com