The reductions in electric current and the power input were confirmed by finite element modeling, and analytically interpreted by the basic laws of electrical resistance, electric power, and heat conduction.
Normalization of input RNA was confirmed by RT reaction of total cellular RNA isolated from 10% of cell extract as was used for IP and subsequent assessment of GAPDH levels.
The number of CFU input per animal was confirmed by ethanol shock of the OD-adjusted suspension, which was then serial diluted 10-fold in TGY and spot plated onto TSA plus SB (5%) to determine the percentage of spores to vegetative cells.
The generated current pulses obviously depend on the rise and fall times and the shape of the input bias U t), as was confirmed by the simulations.
The number of parent bacteria internalized at an input MOI of 10,000 was confirmed visually to be comparable to the mutant strains infected at a MOI of 100 (Figure 6B vs. Figure 4) and internalization of MFN245 at 10,000 MOI remained dependent upon actin polymerization (Figure 6B).
However, input from IFN signaling was confirmed in Stat1−/− cells and by a low level of MED4 and MED7 association after IFNβ treatment alone.
A reduction of β1-integrin total protein expression was confirmed in input samples of equal protein concentration.
Furthermore, although the presence of both promoter regions (proximal and distal) for above genes before the ChIP assay was confirmed by PCR (Input), ChIP assays using pre-immune serum (IgG) did not detect any such amplified bands.
ATP5A antibody was used as a control, and QIL1 knockdown was confirmed by submitting an input sample to SDS-PAGE.
