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By means of a static non-linear coupled mechanical-electrostatic model, has been possible to predict the pressure versus capacitance static characteristic and to tune the geometry of the transducer to reach the required resolution, sensitivity and time response in the appropriate full scale pressure input, ranging from ±250 Pascal.
The dilution series was manually pipetted using RNA input ranging from 0 to 32 ng/μl in the RT reaction (0 to 32 ng/well of cDNA for quantitative PCR).
The simulation procedures were as follows: Initially, the constant external excitatory input ranging from 0 to 5 was applied to the excitatory population in oscillator a (Additional file 1: Figure S2A Left).
The sensitivity of the Streptococcus assays was performed by applying two-step dilution series of the relevant type strains, starting with an input ranging from 0.01 ng to 4.9 fg per reaction.
Gene expression values were quantified relative to a standard curve constructed for each marker transcript with a four-fold serial dilution of cDNA reverse transcribed from QPCR Human Reference Total RNA (Agilent Technologies) at an input ranging from 20 ng RNA to 20 pg total RNA.
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Mechanical threshold of muscle input ranged from 8.8 to 14.7 mN (mean temporal muscle 12.7 mN, neck muscles 13.6 mN).
Since a neural network needs a fixed-sized input ranged from 0 to 1, the bounding boxes are resized and normalized.
Reactions were 10 μl volume with cDNA equivalent to 2 ng total RNA (with the exception of the linearity study, where cDNA input ranged from 2-10 to 2ng/well).
Everything from food packaging to metals processing depends on bulk chemical inputs, ranging from polymers that appear as plastics to adhesives and coatings on automobiles and household goods.
The Accel-NGS 2S DNA Library Kit for Illumina Next Generation Sequencing (NGS) systems provides linear yields from inputs ranging from 10 pg to 1 μg.
This has opened up RWM policy-making and implementation to a wider range of (sometimes contested) expert inputs, ranging across natural and social sciences, engineering, and ethics.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com