Low R values indicate none of the observed trend could be attributed to rDNA copy number variation, and high R values ironically indicate a very high proportion of the trend could be attributed to a function with negligible input of rDNA copy number (because the slopes of the lines are all near zero).
They confirmed that when the sensory input consisted of feedback from the upper body, the motor input consisted of copies of motor commands related to the same body region.
The use of 100 ng of this sample would be equivalent to adding 200 copies of the transgene sequence, considerably more than the experimental input of 10 copies.
The ratio thus generated, which constitutes an index of the percentage of input copies of DNA that are fully methylated at the primer site, was then multiplied by 1000 for easier tabulation (methylation level = target gene/reference gene × 1000).
The ratio thus generated, which constitutes an index of the percentage of input copies of DNA that are fully methylated at the primer- and probe-binding sites, was then multiplied by 1000 for easier tabulation (methylation level = target gene/reference gene × 1000).
The ratio thus generated, which constitutes an index of the percentage of input copies of DNA that are fully methylated at the primer- and probe-binding sites, was then multiplied by 100 for easier tabulation (methylation level = target gene/reference gene × 100).
Pathways detected by GSEA with input of gene expression, copy number variation and methylation data for the PARADIGM algorithm.
The new real time PCR assays showed a good level of repeatability and reproducibility, except for the highest dilutions, with an estimated input of virus DNA copies equal or below 10.
The PMR serves as an index of the percentage of bisulfite converted input copies of DNA that are fully methylated at the primer hybridization sites.
Absolute quantification of viral gene copies was determined from standard curves generated by plotting the log10 of the known input gene copy number of the standard dilution series against the CT value observed in the RQ-PCR analysis.
