Primary recipients of these cultured cells showed that the addition of NGF plus Col 1 to SF plus IL-11 maintained input numbers of DSR-HSCs in the 7-day cultures and produced a 4-fold net expansion of total HSCs (p = 0.48 and < 0.001, respectively; Figure 7D).
We demonstrate an appropriate algorithm for the selection of housekeeping genes, and determine the optimal cytokine induction kinetics and cell input numbers for measuring such responses in the blood.
We observed good agreement between the input number of spike-in RNA molecules and the corresponding FPKM values (Fig. 2d, Additional file 1: Figure S14).
This calculation is comparable to that reported by Nichol et al. [ 23] but provides cost output as a positive savings rather than negative costs. 1. Number of persons immunized (NPI) was calculated from the input number of individuals in the population in an age category, multiplied by the expected coverage in each age category, and totaled across all age categories.
Input these numbers in the following order: Reds: +67, -48, -26, +31.
Input, gene number in input list; BG/Ref, gene number in BG/Ref To identify genes related to metabolic reconfiguration in the different combinations, the MapMan tool was used to select and display the significantly regulated metabolic pathways.
Input, gene number in input list; BG/Ref, gene number in BG/Ref GO terms, such as "biological process", "molecular function" and "cellular component", were identified using AGRIGO (http://bioinfo.cau.edu.cn/agriGO/index.php) with default significance levels (FDR < 0.05).
Briefly, the input number of CD4+ T cells in the enriched sample (90% of total) was calculated by multiplying the number of CD4+ cells in the pre-enrichment sample (10% of total) by a factor of 9.
The binding efficiency of the phage pool was calculated by the ratio of its output phage number to input phage number in each round selection, in order to survey the effect of selection.
