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Lack of precise knowledge of input nuclear physics, referred to as nuclear data, results in simulation uncertainties to which some parameters are highly sensitive.
Input nuclear extract and every second fraction eluting from the column were tested by western blot assays using the Tip60 antibody mixture [ 20] at dilution 1 2000, the anti-Tip48 or anti-Baf53α antibodies [ 58] at dilution 1 500.
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The company also produces some silver, gold and scheelite (tungsten, a super alloy used in stainless steel, as a component in light bulb filaments, as an input in nuclear technology and in medicine)–which should benefit the company as global inflation rises and emerging markets grow.
The Hill-like input-output relationship between Bcd and hb nascent transcripts on the embryonic scale indicates that the mean transcriptional status of a population of individual copies of the hb gene in embryos responds to the graded input of nuclear Bcd concentrations in a highly cooperative manner resembling the DNA binding properties of Bcd measured biochemically [34], [35].
The input and nuclear insoluble fraction (NI) were sheared five times with a 25 G hypodermic needle.
We performed the experiment that Reviewer 1 recommended and measured the mutual information between 1-NM-PP1 input and nuclear Msn2-mCherry for both AM and FM and quantified information loss.
When we carefully quantify Msn2-mCherry dynamics, we observe loss of information between the microfluidic 1-NM-PP1 input and nuclear Msn2 due to variability in Msn2 abundance between cells.
Attention is paid to difficult aspects, e.g. counting efficiency, characterization of irradiation facility; non-ideal behaviour of the epithermal neutron spectrum and consistency of nuclear input data.
Based on our measurements, the mutual information between the 1-NM-PP1 input and Msn2 nuclear concentration is ∼2.06 bits.
Interestingly, based on the input amounts, the nuclear extract contributes significantly more total H1 to the binding reaction than exogenously added rH1 (Panels a, b, lanes 3).
Ikuta showed in 2004 that the ligand-dependent nuclear input of AhR is inhibited by phosphorylation of either Ser12 or Ser36 by PKC.
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