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Coomassie staining shows the input levels of E3s.
Ubiquitination was detected with anti-ubiquitin (Ub) and input levels of the chimeras with anti-GST.
Ubiquitination was detected with anti-ubiquitin (Ub) and input levels of the chimeras with anti-GST Western blotting.
To show relative input levels of E3s, the GST fusion proteins were also loaded separately and stained with Coomassie (panel on the right).
Ubiquitination was detected with anti-ubiquitin (Ub) and input levels of the chimeras with Coomassie staining of separately loaded equivalent amounts of protein.
(C) In vitro ubiquitination reactions with RNF125 and the indicated chimeras, performed as in panel A. Ubiquitination was detected with anti-ubiquitin (Ub) and input levels of the chimeras are shown by anti-GST Western blotting.
Similar(34)
The TR switch is used to protect the receiver from the high-power leakage from the transmitter route with the isolation more than 60 dB, which guarantee its output signal much less than the maximal input level of the LNA.
Since the input level of Rap1-GTP was not sensitive to ERK activity, we placed symbols at a zero input level of Rap1-GTP for clarity.
An input level of 300 pg of total RNA was chosen to allow comparison of both systems.
In the case of a double HPV infection, the fact that the PathogenMip Assay detected and distinguished between two contributing viral loads that differed by 2-logs at an input level of 200ng genomic DNA is quite promising.
At an input level of lower than 250 pg the WT-Ovation One-Direct system resulted in approximately 39 %, 35 % and 18at 100 pg, 50 pg and 10 pg respectively (Table 2).
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CEO of Professional Science Editing for Scientists @ prosciediting.com