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100 ng cDNA was used as input in subsequent quantitative-PCR analysis for either HPRT (TaqMan assay Hs99999909_m1, Applied Biosystems) or Gapdh (Mouse TaqMan Endogenous Control, Applied Biosystems) with iQ Supermix (Bio-Rad) and a Bio-Rad iCycler.
Furthermore, cell-state hierarchies should be statistically supported before being used as input in subsequent analyses.
The processed data when used as input in subsequent processing become raw data to that step.
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The remaining 1 μL of digested DNA was diluted tenfold in water and used as input controls in subsequent amplifications.
Due to the skewness of the distribution of the factor scores (p < 0.001) [ 42], these were dichotomised by the upper quartile, for use as input variables in subsequent statistical analyses.
Stress triaxiality is extracted from the critical element in numerical simulations of smooth tensile tests and used as input in the subsequent unit cell simulations.
The resulting new particle stacks were used as input in the subsequent 3D auto refine and classification processes.
At 48 h post-transfection, the culture supernatant was harvested, clarified, filtered through 0.45 μm filters (Millipore, USA) and tested for luciferase activity to standardize the viral input in the subsequent inhibition analysis.
The genius of the mechanism is that one good idea can be used countless times: The goods and services that one party receives as outputs from transaction can be used as inputs in a subsequent transaction.
It is important to identify partners who share an interest in the priorities selected, and considerable opportunity needs to be provided for input and decision making in subsequent stages of the research.
On the basis of survey responses from 507 academic biomedical researchers, we examine the impact of patents on access to the knowledge and material inputs that are used in subsequent research.
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CEO of Professional Science Editing for Scientists @ prosciediting.com