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This analysis clearly showed the differential clustering of the Metlyl-C immunoprecipitated (IP) versus the input (IN) samples across all 12 arrays, as well as differential clustering of OS (U2OS and MG63) cells in comparison to osteoblasts both before and after normalization.
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We show that the acceleration input in sampled-data control systems excites the mechanical resonance near the sampling frequency in the settling characteristics after the track-seeking control.
A reference gene (ACTB) was used to normalize for DNA input in each sample[ 26].
To normalize for DNA input in each sample, a reference gene (β-actin [ 25]) was used.
A separate assay utilising a methylation-independent primer/probe set on the β-actin gene (ACTB) was used to normalise for the DNA input in each sample.
These techniques involve normalisation to the total RNA input in each sample throughout the analysis, as they aim to identify differentially regulated mRNAs.
NM_001101), were described previously [ 25] and were designed to amplify and detect a region of the gene that is devoid of CpG nucleotides to normalize for DNA input in each sample [see Additional file 2].
After filtering out low expressed genes and genes not enriched by J2-IP over input in either sample (see Materials and Methods and Supplementary Methods), we identified 1,422 genes with increased J2-IP enrichment (P < 0.05, FDR < 0.1) in tdp-1 mutants compared to wild-type, representing about 40% of all transcripts selected by J2-IP.
In IQGAP1 immunoprecipitates, the pERK tERK ratio after phorbol ester stimulation reflects the pERK tERK ratio found in input samples, which is in agreement with IQGAP1 supporting ERK1/2 phosphorylation after phorbol ester stimulation.
As in [6], we first generate a matte interpolation structure from in-sample input images and then use RBF to model the excursion H, for the part of the input image which cannot be explained by a matte model.
The percentage of pure adapter reads was much higher in input samples (~58%) than in CLIP samples (~25%) (Additional File 1 (see Supplementary Material available online at http://dx.doi.org/10.1155/2015/196082)).org/10.1155/2015/196082
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