Sentence examples for input for total from inspiring English sources

Before pre-clearing, 50 μl of the cleared lysate was removed as input for total RNA profiling.

Primer sequences used for the ChIP-qPCR are listed in Additional file 2. The ChIP data are presented as the ratio of percent of input for H4K5ac to percent of input for total H3 normalized to WT levels.

Input data for total column ozone, the aerosol optical depth and vertical profiles of both are identical to the values used in the 1-D-model calculations.

After centrifugation, 500 μl of the lysate supernatant was used as input for the Total Nucleic Acid Isolation Kit (Roche Applied Science) as described by the manufacturer.

500 ng input of total RNA was used for amplification and labeling with the Agilent Low-Input Fluorescent Linear Amplification Kit (Agilent Technologies), according to the manufacturer's guidelines with a slight modification where the transcription (labeling) reactions were done in half the volume.

Table 3 Main input data used for total population calculation S. no.

Because BG levels and endogenous insulin production are related to exogenous calorie and glucose input, we assessed patients for total caloric delivery and intravenous glucose infusion rates (GIR) at the time BG and c-peptide levels were drawn.

For total DNA input, 40 μg were similarly de-crosslinked.

The housekeeping gene β-actin was chosen as a reference for total DNA input measurement.

For total lysate input control, 75 µl of the suspension was mixed with 4×SDS sample buffer and stored separately.

Data were normalised using the internal positive controls included by NanoString Technologies, and the geometric mean of actin, beta 1 (actb1, NM_131031.1) and eukaryotic translation elongation factor 1α, like 1 (eef1a1l1, NM_131263.1) was used to control for total RNA input across samples.