Some of the supernatants were saved as inputs by adding gel sampling buffer and boiled for 5 min. 300 µg of each supernatant was mixed with neutravidin slurry [1∶1 in 1% Triton X-100 IPB (TX-IPB)] and rotated overnight at 4°C.
SVM can deal with the problems that can not be linearly separated in the original input space by adding a penalty function of violation of the constraints to the optimization criterion or by transforming the input space into a higher dimension space.
Adjustment of all the weights, beginning from the output neurons and continuing backwards to the input layer, by adding the corresponding values calculated before and using the equation ( w_{ji} left( {t + 1} right) = w_{ji} left( t right) + gamma delta_{j} left( t right)y_{i} left( t right) ), where γ is the learning rate parameter.
DNA was later purified from input samples by adding proteinase K (USB, Cleveland, OH) to a final concentration of 0.5 mg/ml and incubating at 55°C for 1 hour, prior to purification over a Genomic DNA cleanup & Concentrator column (Zymo Research Corp., Irvine, CA).
Previous approaches in the literature have solved this problem by (1) squaring the system by discarding some inputs or by adding new outputs, or (2) by utilizing some inputs for input/output (I/O) linearization and the remaining inputs for minimizing cost.
Project modifications or construction delays can be evaluated by changing certain input parameters or by adding additional constrains.
The ability to add detail to or modify one's input, for example by adding a room to a drawing, makes it possible to formulate more granular fingerprint-queries and the mental model corresponds better to the designer's own mental model.
The ability to add detail to or modify one's input, for example by adding a room to a drawing, makes it possible to express more granular fingerprint-queries and the mental model corresponds better to the designer's own mental model.
In the first phase, we simplify input feature vectors by adding the frequencies of the paths that include multiple edges to the corresponding paths which consist of only simple edges and then enumerate simple trees for the simplified upper and lower feature vectors.
