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Thus, the optimum combination of heat input and material flow is not met throughout the stir zone.
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We modelled compartments exactly as in Farmer et al. [ 12]; that is, each compartment is a flow reactor in which food is input, and materials leak out at first order.
Key issues in CWM are heat input models and material modelling, which are given special attention in the final sections.
Here, we attempt to simulate the volcanic plume in this sub-Plinian phase of eruption, using the input parameters and material properties listed in Table 1.
For Caco-2 CLIP samples with input RNA as control, we compared four different ranking and normalization strategies, including Raw, AVE-CLIP, AVE-INPUT, and INPUT (Materials and Methods).
Quantitative real-time RT-PCR was performed from input and ChIP material.
Input and eluted material was treated with Pronase (1.5 µg/µl) for 2 hrs at 42°C and de-crosslinked by heating for 12 hours at 65°C.
qPCR was performed using input and ChIP material and enrichment for the region of interest was determined using a variation on the Pfaffl method as described earlier.
Input and immunoprecipitated material were analysed by real time PCR using a series of PCR amplicons and 5'FAM-3'TAMRA probes across the α globin locus [ 26].
The dry matter mass of the input and output materials around pretreatment and enzymatic hydrolysis were recorded.
The total mass and dry matter content of the input and output materials around the pretreatments were recorded.
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