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It was thought that in these experiments it might be possible to see macroscopic growth near the periphery of plates, before there was visible growth in the line connecting the inoculum site to the edge of the plate.
Microscopic examinations of trypan blue-stained leaves for the presence of yeast cells showed C. gattii colonization along wound sites, as well as punctuated colonization across the leaf, even distant from the initial inoculum site and wound (Fig. 1E,1F).
There was a distinctive translucent halo around the inoculum site which showing the positive hemolytic activity.
The distinctive translucent halo around the inoculum site showed positive hemolytic activity, which was measured by using computerised image analysis system (Qwin, Leica, UK).
Although the average signal of all the images acquired from the 10 CFU inoculum site was not found to be statistically significant, removal of negative images resulted in analysis of images with statistically significant signal compared to the negative control (p-value = 0.0026).
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The Log10 average spore inoculum per site was 4.78 (±.51) and the average Log10 recovery per site by Rodac plate sampling was 4.94 (±.74.94
With its early detection and prompt removal of infected hosts, the Oregon SOD eradication program has produced a landscape distribution of disease resulting predominantly from the long distance (100 m to 4 km) dispersal of inoculum between sites.
Each pepper leaf was inoculated with five 2-μl drops of a zoospore suspension of P. capsici along the edge of the leaf and a 10-μl drop of molten agar was added to each inoculation drop to fix the inoculum on the target site.
The exclusive expression of 5'Nuc in the salivary gland tissue suggested that the activity is primarily required in the saliva inoculum at the bite site.
Repeated injections of fibroblasts conditioned medium, at the site of inoculum of tumour cells also enhanced tumour growth, suggesting the involvement of soluble factors secreted by fibroblasts.
The inoculum applied to each site was adjusted such that 4 to 5 log10CFU/cm2 were recovered from the positive control specimens after desiccation (original culture approximately 9 log10CFU/mL).
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