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Only one conserved the original inoculum sequence.
There were no significant deviations from the inoculum sequence observed for all the genes encoding internal proteins and the neuraminidase gene in either directly infected or TC animals.
The number of synonymous substitutions per synonymous site (dS) and the number of nonsynonymous substitutions per nonsynonymous site (dN) between the viral sequence obtained from each animal's circulating virus and the inoculum sequence (SIVmac239) were estimated using the method of Nei and Gojobori [69], and the results were analyzed as previously described [68], [70].
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These researchers reported a SMY of 182.9 ± 16.9 NL CH4 kg−1 VS fed at organic loading rate (OLR) of 3.0 g TCOD kg−1 inoculum day−1 in sequence batch reactor (SBR).
In HpSVd-grapevine, all 5 cDNA clones sequenced from the inoculum had the same sequence (HpSVd-g or hKY04-1 in Table 1).
There were no sequences found in group a of the NC10 phylum, unlike the results of the original inoculum where 14 sequences clustered in group a (Fig. 1a).
This paper reports the start-up phase of a novel psychrophilic (20 °C) dry anaerobic digestion of dairy manure with bedding fed at 35%% total solids and an organic loading rate of 3.0 g total chemical oxygen demand kg−1 inoculum day−1 in anaerobic sequence batch reactors.
In HpSVd-hop, all 5 cDNA clones used for the inoculum showed the same sequence as the HpSVd-hop, hJType (Table 1).
Note that, since the virus evolved independently in each monkey, comparisons of viral sequences with the inoculum are both statistically and phylogenetically independent [71].
The HA gene sequencing of virus inoculum revealed that it differs from the original virus, A/California/07/2009 (GenBank accession numbers: FJ966974, FJ969540 and FJ981613)[2] through the presence of a mixture of two amino acids at HA positions 225 and 226 (H3 numbering, [27]), D/G and R/Q, respectively.
Sequences were compared to the original sequences used for the inoculum.
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