After 1-h adsorption of inoculum at 37 °C, inoculum was removed and overlaid with maintenance media containing 0.9% agarose (BD BaculoGold Plaque Assay Agarose).
During experiments with different G. sulfurreducens inoculum concentrations, the concentrated inoculum solution was diluted using fresh co-culture medium prior to inoculation.
Result showed significantly higher basal expression levels for NanA and HylA in T inoculums than in O inoculum.
The experiment was a complete factorial design with 11 recently-collected soils and two mycorrhizae treatments (either inoculated with fresh "live" mycorrhizal inoculum or without inoculum).
The incidence of grey mould in sweet pepper and tomatoes treated with the heat-inactivated inoculum or no inoculum (control) was approximately 80% on the flowers and leaves.
Culturing T. lanuginosus D2W3 on sorghum straw and optimizing other culture conditions (media types, particle size of carbon source, inoculum level, inoculum age and additives), yielded increased levels of xylanase (39,726 units/g dry substrate).
For this, Plackett Burman design was constructed with 11 process variables including media components (wheat bran, peptone, xylose, KNO3 and yeast extract), incubation conditions (shaking conditions, incubation temperature and incubation time), pH, inoculum age and inoculum size.
Gas production (ml/g organic matter) with S1 was the highest, avoiding to be in excess for microbial colonisation and activity and inoculum I20 limited inoculum contribution by self-fermentation; they were hence considered for further trials.
At the above optimal conditions, the model predicts a maximum relative surfactin concentration of 58 59 CMC−1, mainly due to a strong interaction between primary inoculum size and secondary inoculum age.
CCD predicted the maximum xylanase activity (IU/ml) at the optimum conditions of xylose (0.1 g/50 ml), incubation time (22 h), yeast extract (0.1 g/50 ml), peptone (0.1 g/50 ml) and temperature (30 °C) while the fixed variables were wheat bran (0.3 g/50 ml), KNO3 (0.1 g/50 ml), inoculum size (0.5 ml), inoculum age (16 h) and shaking at 120 rpm.
