Therefore, the optimal inoculum dose was set as 2%% in the follow-up experiments.
Lactonic SL production and proportion of lactonic SL to total SL increased with increasing inoculum dose, which demonstrated that high inoculum dose is more suitable for lactonic SL production in RSHH medium.
As the increase of inoculum dose, the biomass was increased and the final broth pH was decreased.
The optimum inoculum dose in RSHH medium dose for total and lactonic SL production was 2 and 4%%, respectively.
Several parameters including substrates, inoculum dose, yeast extract concentration and pH regulator was optimized to attempt to reduce the cost and make it more economical.
The influence factors of inoculum dose, yeast extract concentration and pH regulators (chemical regents used for adjusting/influencing pH) was investigated and discussed.
Then, effects of different seed inoculum dose, yeast extract concentration and pH regulator on SLs production and lac SL/tot SL ratio in RSHH fermentation medium was investigated.
In view of this, the effects of inoculum dose, nitrogen source (yeast extract) and pH regulator additions on sophorolipid exting was studied systematically.
The optimized conditions included incubation time (a), temperature (b), pH (c), C/N ratio (d), inoculum dose (e), rotary speed (f), and carbon source (g).
Effects of the inoculum dose on SL production were shown in Table 3, glucose in RSHH could be applied directly for SL production by W. domercqiae.
Inoculum dose, yeast extract concentration and pH regulator made obvious influence on fermentation parameters, especially on final broth pH and SLs production.
