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Moreover, cells used for the inoculum (day 0) and at days 3 and 6 of cultivation in the bioreactor, were characterized using immunofluorescence tools and the neuronal differentiation potential evaluated (see below).
This labeling pattern was similar to the cell inoculum (day 0).
The microbial composition of the inoculum (day 0) and the four reactors at day 59 was studied and compared using 454 FLX Titanium pyrosequencing.
Cells used for the inoculum (day 0) and from day 3 of the bioreactor expansion culture were dissociated into single cells and analyzed by flow cytometry.
To four 10-L continuously stirred tank reactors, designated R1, R2, R3, and R4, were added 2.55 L inoculum (day 0).
Similar(54)
OLR was expressed in g of TCOD fed per kg of inoculum per day, and g of total VS fed per kg inoculum per day.
The supernatants from BGM, CV-1, MEK, Vero, and Vero E6 cell lines yielded Ct values 12 17 units lower than the initial cell-free inoculum by day 4 after infection.
Bacterial suspensions were stored at 4°C and used as an inoculum at the day of preparation.
The inoculum sizes at Day 0 were comparable for both strains and were 2 × 10 ± 2 × 10.
Whole-blood CQ levels, at least in mice malaria models, could be affected by differences in hematocrit and parasitemia, both of which can vary significantly depending on parasite species, initial inoculum load and day postinfection.
Moreover, they demonstrated successful operation at TS of 27 % and organic loading rate (OLR) of 3 g total chemical oxygen demand (TCOD) kg−1 inoculum day−1 for 273 days with an average SMY of 182.9 ± 16.9 NL CH4 kg−1 VS fed (Massé and Saady 2015c).
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