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The effect of the artificial inoculation type and initial microbial load, however, has not been thoroughly assessed in the context of inactivation studies.
Plants were grown in a randomized complete block design until inoculation at which point plants were grouped per inoculation type (mock, PVYO, PVYNTN).
The experiment followed a completely randomized design with the three replicates as blocks and a full factorial treatment structure consisting of three treatment factors: hai (12 hours), genotype (resistant or susceptible), and inoculation type (ASR or mock).
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One of 10 old Tax transgenic mice (10%) developed weak arthropathy (total arthritis score 2) before inoculation with type II collagen; however, the arthropathy observed in that mouse did not become more severe after inoculation with type II collagen, and none of the other mice developed arthropathy (Figure 5B).
In the inoculated plant samples, time course expression values (-ΔCt) were calculated and tested for statistical significance using ANOVA with the following variation factors: days post inoculation (dpi), type of interaction (compatible vs. incompatible), and P. halstedii race.
To a lesser extent, other factors such as a high inoculum size, use of MRS as carrier for the starter culture, inoculation time, and type of acid to correct the pH had also an overall significant effect on the final acidity or total corrected acidity.
12 h post-inoculation wild type spores had germinated and begun to form mycelia whereas ΔssrA spores remained ungerminated (Figure 6A).
Starting 6 weeks prior to prion inoculation, wild-type mice (C57BL/6) were immunized and repeatedly boosted with generic antigens unrelated to prions, with the goal of achieving sustained activation of immune cells and activation-related morphological changes within lymphoreticular organs.
Cattle experimentally infected by intracerebral inoculation with L-type or H-type BSE present with two clinical phenotypes, either dull or nervous forms, which may be less clinically overt than classical BSE although difficulty in rising is consistently displayed.
To examine which cell types are affected by Pst virulence effectors, DIR1-GUS expression in the DIR1pro DIR1-GUS-29/ DIR1pro DIR1-GUS-29/ DIR1pro DIR1-GUS-29/ dir1-1 dir1-1ype Pst and a hrpA Pst mutant that does not make the major pilineprotein, HrpA and therefore cannot form the T3SS Hrp pilus or deliver effectors into the plant cell [ 36].
Relative abundances of Bacteria, Eucarya and Archaea were not affected by either inoculation or buffer type.
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