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The different tissue inoculation ratios were tested to achieve the best growth of G. tinctoria shoots and hairy roots in the co-culture system.
As for the rapamycin production, the batches with 10 and 5%% inoculation ratios had similar rapamycin concentrations, which were higher than that obtained with the 2 % batch.
Due to the effects of high shear force resulting from the agitation, a lot of mycelium were observed in the broth among all batches with various inoculation ratios.
No significant differences in the morphology were observed among all batches with different inoculation ratios, as all were in loose pellet form, indicating that the inoculation ratio would not affect cell coagulation in the agitation tank.
The inoculation of the dark chamber was a constant value of 1.6 mg E. harbinense B49, while the inoculation of the photo chamber was 16, 32, and 48 mg, corresponding to inoculation ratios of dark to photo chamber of 1 10, 1 20, and 1 30.
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The total organic matter content of each group was controlled at 6 gVSS, in which 0.6 gVSS was inoculated sludge (inoculation ratio 10%%) and 5.4 gVSS was WAS (without conditioning).
Cells were harvested by centrifugation, washed with PBS and used to inoculate fresh TSB with an inoculation ratio of 1∶1.
A defined inoculation ratio of 2, 5 and 10%% (v/v) of seed medium was then inoculated into a 15-L agitation fermentor.
Therefore, the effects of inoculation ratio on rapamycin production were examined in this study.
An inoculation ratio of at least 5%% is thus suggested to achieve relatively good rapamycin production.
After 100 days, all three inocula had produced the same amount of methane, for the same inoculation ratio.
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