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Recording was completed when inoculation points on the same media plate touched (22 days).
Four inoculation points were marked at evenly spaced distances (ca. 10 cm) in a square pattern with fine mounting pins.
Venom inoculation points were in the lower limbs in 80% of cases, followed by upper limbs (20%).
Three adjacent needle fascicles were inserted into a 15 mL Falcon tube containing 15 mL of freshly prepared zoospore suspension with no more than one inoculation per twig of needles to avoid cross contamination between inoculation points.
However, we did not observe the typical straight line of zygospores and instead found them diffusely distributed across the plate within ca 4 to 5 cm of the inoculation points.
Similar(55)
A volume of 50 μL was then applied to each respective inoculation point.
A = 1st fragment of 5 cm (from the inoculation point) of infected leaves.
At harvest, samples of bark (if present) and sapwood were taken from the inoculation point and at a distance 5 mm from the inoculation point using a number-3 cork-borer.
D = 4th fragment of 5 cm (from 15 to 20 cm to the inoculation point) of infected leaves.
C = 3rd fragment of 5 cm (from 10 to 15 cm to the inoculation point) of infected leaves.
B = 2nd fragment of 5 cm (from 5 to 10 cm to the inoculation point) of infected leaves.
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