The term "inoculation loop" is a correct and usable phrase in written English.
Generally, it is used to describe a situation in which a person or group is exposed to a risk or danger or made vulnerable to a threat in order to help them develop immunity or resistance. For example, "Instructors at the self-defense class take an inoculation loop approach to training, exposing students to mock attacks in order to help them prepare for and learn how to handle real-world encounters."
The samples were inoculated on the agar plates and diluted by an inoculation loop obtaining 3 levels of dilution on the agar plate.
Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Klebsiella pneumoniae were procured from Doctors Diagnostic Centre, Tiruchirappalli and subculture in nutrient broth with the help of sterile inoculation loop.
The spores were dislodged using a sterile inoculation loop under strict aseptic conditions, and the number of spores in the suspension was counted using a Neubauer chamber.
Some EPS confer on LAB a "ropy" character that can be detected in cultures that form long strands when extended with an inoculation loop.
Conidial suspensions were prepared by adding 10 ml of 0.1% Tween 80 (AppliChem) into the 4-week-old Petri dishes, and the conidia were taken from the agar surface, using a sterile inoculation loop to dislodge.
For the inoculum preparation, an ampule of API NaCl 0.85 % (2 ml) was selected and 1 4 colonies of bacteria were picked up using inoculation loop from the agar plate and then suspension was prepared with the turbidity equivalent to 0.5 McFarland.
DNA was precipitated with two volumes of 99% ethanol and fished out with inoculation loops, washed in 70% ethanol and dissolved in 1xTE buffer containing 10 µg/ml RNase A at 55°C overnight.
Bacterial inoculum was retrieved from the culture with 2 inoculation loops.
DNA was precipitated with two volumes of 99% ethanol and fished out with inoculation loops, washed in 70% ethanol and dissolved in Tris-EDTA buffer containing 10 mg/ml RNase A at 55°C overnight.
The cells were then resuspended in thirty microliters of 0.45% NaCl solution with or without 1 g/litre ertapenem (MSD, USA) in 1.5-mL Eppendorf tubes and the amount of bacteria filled a 1- μL-inoculation loop (corresponding to 3 6 × 10 cells, 1 mL of a McFarland 1-2).
The pigs were euthanized by an overdose of pentobarbital 15 16 h after inoculation of the loops.
