Samples were taken at the time of inoculation (0-hr time point) and at 2, 4, and 22 hr after inoculation and were visualized by differential interference contrast (DIC) microscopy.
However, by 24 hrs after inoculation, animals inoculated with either 923Δagr or 923Δsae had recovered and appeared normal.
Regarding the ADSCs, after the first inoculation of 5 7 hr, some cells began to adhere and exhibited elongated pseudopodia, thereby contributing to a spindle-like appearance.
The majority of ESTs representing 13 of the genes (PAL, C4H, 4CL, CHS, CHR, CHI, IFS, IOMT, IFR, CCR, CAD, COMT and CCoAMT) hybridized complementary RNAs at increasing amounts from 2 to 8 hrs post inoculation in concurrence with the HR as indicated by the ratios marked in bold type.
Specifically, the expressions of SlMKK2 and SlMKK4 in B. cinerea-infected plants showed approximately 45 and 8 folds of increases over those in the mock-inoculated plants at 12 hr after inoculation.
For i.v. inoculated mice, blood were collected at 84 hr post inoculation (∼ median survival time) and dilution plated.
Rosette leaves of 4-week old plants, mock treated or spray inoculated with spores of B. cinerea 2100, were collected at 14 hr post inoculation and used to perform ChIP-seq.
As shown in Figure 1, the expression level of SlLapA in B. cinerea-inoculated plants increased significantly, leading to 50 folds of increase at 24 hr post inoculation (hpi) and >300 folds of increase at 48 hpi relative to those in the mock-inoculated plants, suggesting that our inoculation assays were appropriate for further analysis of the expression patterns of SlSRs in response to B. cinerea.
The spleen bacterial burden in i.p. inoculated mice were essentially the same between SU6656-treated and the control mice at 72 hr post inoculation (∼ median survival time) (Fig. 6, D).
route and bacterial dissemination was examined at 72 hr post inoculation.
In fact, a study performed in 1975 demonstrated that V. cholerae administered to mice orally was killed in the small intestine within 2 hr after inoculation [14].
