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Samples (40 mL) of each culture, taken at 24, 48, 72, 96 and 120 h post inoculation (h pi) and centrifuged at 4500×g for 20 min in a Sorvall RC6-Plus centrifuge.
Table 8 Time required for zoospores of Phytophthora capsici to infect pepper leaves after inoculation Time of inoculums removal after inoculation (h) Infection rate Lesion size (mm) 0 0 0 2 0 0 4 0 0 8 100 15 16 100 15 32 100 15 Control (water) 100155.
At 6, 12, 24 and 48 hours after inoculation (h p.i). the abaxial epidermis of at least 20 primary leaves was peeled and directly frozen in liquid nitrogen.
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The roots of rice plant grown in SAP amended with 1.2%% biochar or the roots of untreated control plant roots were collected just before inoculation (0 h) and 24 h after inoculation with the RKNs.
200 μL aliquots were taken immediately after inoculation (0 h), and every 24 h after that, for cell counts and TAG quantification.
six rats from each group were euthanized with by cervical dislocation after anesthesia by 2 % pentobarbital sodium injection prior to inoculation (0 h) and then at 6, 12, 24, 48, 72, and 96 h post inoculation (pi).
The endemic status of the disease was determined by calculating the inoculation rate (h) of each group.
To determine the PHB content a 5 mL sample was taken immediately after inoculation (0 h).
RFP expressing B31 andand GFP expressing Δp66 B. burgdorferi were imaged at the site of inoculation 1 h post-intradermal inoculation.
MPK25 was significantly downregulated in response to inoculation after 24 h and 48 h, and its expression recovered to high levels at 96 h post-inoculation.
The OD600 was measured immediately after inoculations and at 24 h after inoculation with a microplate reader (Model EL808, BioTek Instruments, Winooski, VT).
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