Exact(55)
Six plants per treatment (treatment = mock or PVY inoculation) for each variety were inoculated.
To monitor the success of the inoculations, additional inoculated and mock-inoculated plants were kept for 9 days after inoculation for continuous observation.
The eggs were candled daily, and allantoic fluids from 3 inoculated embryos were collected 48 h after inoculation for RT-PCR amplification.
It followed an inoculation for smallpox; it did not precede the inoculation.
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The mice were then dissected on Day 3 post-inoculation for bacterial enumeration.
This was typically 4 5 days post inoculation with 0.001 p.f.u./cell for ORF75c+ viruses and approximately 2 weeks post-inoculation for ORF75c− viruses.
Groups of mice were then sacrificed on day 3 or 7 post-inoculation for the enumeration of bacteria in the lungs.
Tracheas were removed from three randomly selected chickens from each group at 4 and 7 days post-inoculation for assessment of ciliary activity.
Mice were monitored for 48 hours post-inoculation for procedure-related adverse events.
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