Mice (n=8) were imaged 15 days after inoculation, using INVEON microPET/CT device (Siemens), after IV injection of 0.36±0.04MBq/g of [18F]MEL050.
Currently, it is unknown if H. capsulatum is able to form biofilms in its filamentous form, which could contaminate hospital environments, medical devices, and supplies, facilitating the direct inoculation of the infective form through cross-contamination.
An experimental model of device-related infections was developed by direct inoculation of Staphylococcus aureus into the canal of both femurs of 34 rats.
This review focuses on the construction of animal models for percutaneous-device-related infections, and specifically the role of inoculation of bacteria in such models.
At different times after inoculation, cultures were subjected to cell counting using a CASY cell counting device (Schärfe system).
But on certain occasions, the presence of S. epidermidis as a contaminant of medical devices, or breach of the skin by trauma or inoculation needles, makes it emerge as a causative agent of infections [ 1].
That device, an air-powered, pistol-shaped instrument invented in 1960, made possible mass inoculation on a far larger scale than ever before and proved vital to the eradication of smallpox, then the planet's deadliest pathogen.
The inoculation of bacteria is critical to obtain an animal model for standardized studies for percutaneous-device-related infections.
The design of percutaneous devices, susceptibility of various animal species, bacterial strains, amounts of bacteria, method of inoculation and methods for subsequent evaluation of the infection are discussed in detail.
In his excellent book Aberrations of Mourning: Writing on German Crypts, he points to the advent in the west of recording devices such as phonographs and gramophones before infant mortality rates had been reduced by mass inoculation, even among the better off.
