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This inoculation cycle was cycle 1.
Thus, it is unlikely that all Coulombic recoveries larger than 100% were due solely to organic matter carryover from the inoculation cycle.
Batch cycles typically lasted for 4 5 weeks for all materials except platinum, which required 2 3 weeks per cycle except during the batch cycle following the inoculation cycle (cycle 2, Table S1, Supporting Information).
Organic matter remaining from the inoculation cycle could have contributed to the high rate of methane production, especially for brushes that might capture more material than flat electrodes that have much lower surface area.
Although the rates of methane production by the open circuit reactors (controls) were similar to those of the reactors with set potentials during the inoculation cycle, there was no evidence of methane production following this first cycle (cycle 2; Figure S1, Supporting Information).
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Methane production was observed in all reactors following inoculation (batch cycle 1; Figure 1).
After inoculation, each cycle consisted of 40 min of filling, about 240 min of aeration, 60 min of settling and 30 min of effluent withdraw.
This inoculation-isolation cycle was repeated four times.
The cluster analysis revealed that the treatment order did not have an impact on the gene expression profiles, with samples not clustering according to treatment group, but 19 out of the 20 samples clustering by inoculation status and cycle stage, with one post-inoculation dioestrus sample clustering with the pre-inoculation samples (Additional file 2: Figure S2).
10 μ g kg−1 body weight CF101, given orally once daily (initiated 7 days after tumour inoculation), chemotherapy – one cycle of intraperitoneal 5-FU at 20 mg kg−1, given once a day for five consecutive days (initiated 1 day after tumour inoculation), 5-FU+10 μ g kg−1 body weight CF101 (as detailed above).
Continuation therapy consisted of short cycles of intermittent chemotherapy and BCG inoculation or long cycles of intermittent chemotherapy +/- BCG.
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