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These included using an inoculating loop to disrupt the previously inoculated surface of the plant tissue in the presence of ∼2 ml sterile water.
It consisted in inoculating pipes with a highly diverse microbial consortium (urban wastewater treatment plant), and trickling a feeding solution containing a safe and soluble reduced sulfur source, thiosulfate, over the inoculated surface in order to select a sulfur-oxidizing activity.
The inoculated surface of the filter paper was then placed in contact with the leaf.
Topographical features of the inoculated surface [ 40], the availability of nutrients [ 40, 57] and plant exudates [ 40, 43, 57- 59] were discussed to be influencing factors for development and morphology of compound appressoria from R. solani.
Similarly for the agar plates and porcine skin pieces used for sponge based applicator, the sponge post wetting with liquid 5% Betadine is gently rubbed on the inoculated surface in regular motion.
Each disinfectant was sprayed to completely wet the inoculated surface and allowed to remain in contact with the surface until completely dried (~10 minutes) with no surface wiping or allowed to remain in contact with the surface for 3 minutes followed by a manual wipe using sterile gauze that was pre-moistened with the same disinfectant.
Similar(54)
Sterile biopsy punches (4 mm; VWR International, Radnor, PA) were used to obtain samples from inoculated surfaces.
The RODAC contact plates were pressed onto inoculated surfaces for 5 s while avoiding air bubbles between the surface and RODAC plate.
Organisms were not inoculated onto surfaces for these experiments.
We found that the Tru-D device was effective in killing C. difficile spores, MRSA, and VRE inoculated onto surfaces in the laboratory and in hospital rooms.
C. difficile spores (in ATS as an organic challenge) were used to inoculate the surface of a toilet seat (4 Log10 spores per site) and dried overnight.
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