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This subtractive hybridization was performed using sample cDNAs (tester) from inoculated plants, which was subtracted with cDNAs (driver) from non-inoculated plants.
In Fol + Th + SA treated plants, it was 132.92% lesser than pathogen inoculated plants (Fig. 5a).
Water inoculated plants served as controls.
SiRNAs were analysed in PPV inoculated plants.
Mock inoculated plants were buffer-rubbed.
Of the 210 inoculated plants 86.67% showed disease symptoms.
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Non-inoculated plants served as control at the time of inoculation.
The number of emerged plants was significantly different between the non-inoculated plants and the S. enterica-inoculated plants (χ2=9.231; P=0.026).
Non-inoculated plants showed marginal EM colonization (3%).
Mound-inoculated plants that were drought stressed also produced more than twice as many leaves as non-inoculated plants.
Differentiation of leaf metabolomes was nevertheless not total for all conditions (non-inoculated plants of fields FC and C, or inoculated and non-inoculated plants of field C have, for example, overlapping distribution ellipses on Fig. 4A).
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